Genomic targeting with purified Cre recombinase
نویسندگان
چکیده
منابع مشابه
Genomic targeting with purified Cre recombinase.
Purified Cre recombinase protein introduced directly into cultured mammalian cells by lipofection catalyzes both site-specific chromosomal integration of a co-transfected lox targeting vector and precise excision of genomic DNA flanked by directly repeated lox sites. This procedure eliminates the need to transfect cre expression plasmids to activate recombination at lox sites. We used this simp...
متن کاملNuclear targeting determinants of the phage P1 cre DNA recombinase.
The Cre DNA recombinase of bacteriophage P1 has become a useful tool for genomic manipulation in mice and other eukaryotes. Because Cre is of prokaryotic origin, the 38 kDa protein has been presumed to gain access to the eukaryotic nucleus simply because it is sufficiently small to pass through the nuclear pore by passive diffusion. Instead, we show here that Cre carries nuclear targeting deter...
متن کاملMutants of Cre recombinase with improved accuracy
Despite rapid advances in genome engineering technologies, inserting genes into precise locations in the human genome remains an outstanding problem. It has been suggested that site-specific recombinases can be adapted towards use as transgene delivery vectors. The specificity of recombinases can be altered either with directed evolution or via fusions to modular DNA-binding domains. Unfortunat...
متن کاملCre/lox-Recombinase-Mediated Cassette Exchange for Reversible Site-Specific Genomic Targeting of the Disease Vector, Aedes aegypti
Site-specific genome modification (SSM) is an important tool for mosquito functional genomics and comparative gene expression studies, which contribute to a better understanding of mosquito biology and are thus a key to finding new strategies to eliminate vector-borne diseases. Moreover, it allows for the creation of advanced transgenic strains for vector control programs. SSM circumvents the d...
متن کاملTargeting Cre recombinase to specific neuron populations with bacterial artificial chromosome constructs.
Shiaoching Gong,1 Martin Doughty,1 Carroll R. Harbaugh,4 Alexander Cummins,4 Mary E. Hatten,2 Nathaniel Heintz,3 and Charles R. Gerfen4 1Gene Expression Nervous System Atlas Project, 2Laboratory of Developmental Neurobiology, and 3Laboratory of Molecular Biology, Howard Hughes Medical Institute, The Rockefeller University, New York, New York 10021, and 4Laboratory of Systems Neuroscience, Natio...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1993
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/21.9.2025